Until now PCR instruments have relied on Peltier elements to heat and cool an aluminum or silver block, accommodating 96 or 384 polypropylene tubes, holding PCR-mixes. Using this technology PCR times, for 30 cycle, 3-temperature reactions, are usually between 50 and 150 minutes. Now, NEXTGENPCR is redefining fast. The thermocycler introduces a new way to heat and cool the samples instantly, with virtually no ramp rates, losing no time getting the samples to the desired temperature. The user can go from melting to annealing in less than 0.1 second. For a 100 base pair fragment, total reaction time is as low as 2 minutes, with 700 base pairs being amplified in 10 minutes. Adding NEXTGENPCR into workflows results in higher sample throughput. Using optimized applications, more than 10 full plate experiments per hour become possible. Operated at capacity this would mean 80, 384-well plates per day, resulting in more than 30,000 datapoints per day; far beyond the capabilities of common thermocyclers.
During this presentation, Gert will focus on the technology behind NEXTGENPCR. Special attention will be paid to publications detailing 2 minute PCR data and how NEXTGENPCR amplified all 29 fragments of the BRCA1 gene in less than 10 minutes for subsequent Sanger sequencing.
Gert de Vos
Director, Molecular Biology Systems B.V.View presenter info
Gert de Vos is the co-inventor of NEXTGENPCR and Director at Molecular Biology Systems of Goes, Netherlands. Gert has Master’s Degrees from Leiden University in Biology & Biophysics and Physics. After teaching physics in Curaçcao, Gert returned to the Netherlands and embarked on a career as an entrepreneur and inventor in life sciences and medical device technology.